Repurposing the anticancer drug cisplatin with the aim of developing novel Pseudomonas aeruginosa infection control agents

• Mingjun Yuan,
• Song Lin Chua,
• Yang Liu,
• Daniela I. Drautz-Moses,
• Joey Kuok Hoong Yam,
• Thet Tun Aung,
• Roger W. Beuerman,
• May Margarette Santillan Salido,
• Stephan C. Schuster,
• Choon-Hong Tan,
• Michael Givskov,
• Liang Yang and
• Thomas E. Nielsen

Beilstein J. Org. Chem. 2018, 14, 3059–3069, doi:10.3762/bjoc.14.284

• experiments, transcriptomic profiling showed upregulation of the recA gene, which is known to be important for DNA repair, implicating that cisplatin could interfere with DNA replication in P. aeruginosa. Cisplatin treatment significantly repressed the type III secretion system (T3SS), which is important for

• infections. Keywords: biofilm; cisplatin; Pseudomonas aeruginosa; resistance; type III secretion; Introduction Pseudomonas aeruginosa is a leading nosocomial pathogen which causes, among others, corneal, chronic otitis media, urinary tract (UTI) and respiratory tract infections [1]. P. aeruginosa is also

• targeting important components of the immune system, such as the type III secretion systems (T3SS), which was shown to be associated with poor clinic outcomes in patients with lower respiratory infections [8] and ventilator-associated pneumonia [9]. Identifying antimicrobial compounds which actively target

Pathoblockers or antivirulence drugs as a new option for the treatment of bacterial infections

• Matthew B. Calvert,
• Varsha R. Jumde and
• Alexander Titz

Beilstein J. Org. Chem. 2018, 14, 2607–2617, doi:10.3762/bjoc.14.239

• systems exist in bacteria [65] and the Gram-negative specific type III secretion system (TTSS) is a focus of current research. TTSS is a major virulence determinant in a number of pathogens, including P. aeruginosa. In TTSS, toxins are secreted from the bacterial cytosol across the bacterial membranes and

Impact of Pseudomonas aeruginosa quorum sensing signaling molecules on adhesion and inflammatory markers in endothelial cells

• Carmen Curutiu,
• Florin Iordache,
• Veronica Lazar,
• Aurelia Magdalena Pisoschi,
• Aneta Pop,
• Mariana Carmen Chifiriuc and
• Alina Maria Hoban

Beilstein J. Org. Chem. 2018, 14, 2580–2588, doi:10.3762/bjoc.14.235

• vascular endothelial cadherin (VE-cadherin) is the major structural component of endothelial AJ with a pivotal role in endothelial barrier integrity. LasB protease released by P. aeruginosa during infection determines VE-cadherin cleavage and facilitates type III secretion system toxicity in endothelial

• 2010 that ICAM-1 expression is modulated by ExoU cytotoxin injected via the type III secretion system, being involved in endothelial damage and bacterial dissemination [24]. Inflammatory markers Proinflammatory cytokines and chemokines such as IL-1α, IL-1β, IL-6 and TNFα are produced by vascular cells